Cunninghamella echinulata produced from soybean wastewater cleanly harvests oleaginous yeasts in soybean oil refinery effluent by efficient adhesion and improves microbial lipid quality

The oleaginous yeast Trichosporon fermentans can degrade oils and fats in soybean oil refinery effluent (SORE) accumulate intracellular lipids. Effective harvesting techniques for yeasts improve their utilization efficiency. Considering its excellent adhesion properties, Cunninghamella echinulata, a lipid-producing fungus, was introduced to harvest the SORE via two-step process. Compared with C. echinulata cultured traditional medium, namely, potato starch wastewater (PSW), produced from (SW) achieved better yeast, reaching 95.24%. Based on kinetic thermodynamic analysis, it found that are gathered by mycelium pellets of physical adsorption, process is controlled liquid film diffusion intraparticle diffusion. porosity these as high 79.3 ± 0.5%, which contributed significantly echinulata's ability adhere yeasts. However, when pH between 6 7, an electric neutralization effect responsible higher SW T. compared PSW. Because microbial lipids rich unsaturated fatty acids (UFAS), highest proportion UFAS reached 79.8% total extracted products. used yeasts, efficient represented cleaner improved quality